Chiefland is a city in Levy County, Florida, United States. The population was 2,316 at the 2020 census, up from 2,245 at the 2010 census. It i…Chiefland is a city in Levy County, Florida, United States. The population was 2,316 at the 2020 census, up from 2,245 at the 2010 census. It is part of the Gainesville, Florida Metropolitan Statistical Area. Chiefland calls itself "The Gem of the Suwannee Valley" and was incorporated in 1913.